Serum-free Media

Many of Athena’s Cell Culture Products are Serum-Free Media developed for use in mammalian tissue culture research. Athena provides an array of serum-free media in addition to several supplementary products to aid in the growth and preservation of mammalian cell lines. The above symbol indicates our serum-free products. Viral, bacterial, and fungal contamination of serum is common and presents serious safety hazards for manufacturers of biopharmaceuticals. The use of serum-free media significantly reduces the possibility of this potential contamination.

BRFF-BMZERO™

BRFF-BMZERO™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing human breast cell lines. BMZERO™ was developed and has been extensively used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.
Order # Product Name Type Unit Price
0401 BRFF-BMZERO Liquid (ready-to-use) 500mL $130.00
datasheet_bmzero_v1.pdf
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File Type: pdf
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Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4 - 6 weeks
pH 7.3 ± 2
Osmolality 270 - 300mOsM

BRFF-EPM2™

BRFF-EPM2™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors optimized for culturing human esophageal epithelial cell lines. EPM2™ has been used for culturing epithelial cells from explant cultures of human skin as well as for culturing a number of human cancer cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.
Order # Product Name Type Unit Price
0402 BRFF-EPM2™ Liquid (ready-to-use) 500mL $130.00

Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4 - 6 weeks
pH 7.3 ± 2
Osmolality 270 - 300mOsM

​BRFF-HPC-1™

BRFF-HPC-1™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements, growth factors and dihydrotestosterone, designed for establishing new cell lines from human prostate tissue. HPC-1™ was developed and has been extensively used for establishing and maintaining epithelial cell lines from both benign prostatic hyperplasia and prostatic carcinoma. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.
Order # Product Name Type Unit Price
0403 BRFF-HPC1™ Liquid (ready-to-use) 500mL $124.30
datasheet_hpc1.pdf
File Size: 159 kb
File Type: pdf
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Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4 - 6 weeks
pH 7.3 ± 2
Osmolality 270 - 300mOsM

​BRFF-P4-8F™

BRFF-P4-8F™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing immortalized normal prostatic cell lines such as 267-B1.1 P4-8F™ supports the growth of certain established human prostatic cancer cell lines such as PC-3. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.
Order # Product Name Type Unit Price
0404 BRFF-P4-8F™ Liquid (ready-to-use) 500mL $130.00

Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4 - 6 weeks
pH 7.3 ± 2
Osmolality 270 - 300mOsM

DMEM / F12 Serum-Free Medium

DMEM/F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham’s F12 media supplied complete and ready to use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types. DMEM/F12 does not contain phenol red or antibiotics.
Order # Product Name Type Unit Price
0410 DMEM / F12 SFM Liquid (ready-to-use) 500mL $76.60
datasheet_-_0410_dmem-f12.pdf
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Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4 - 6 weeks
pH 7.3 ± 2
Osmolality 270 - 300mOsM

IMDM Serum-Free Medium

Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium. It is supplemented with bovine pituitary extract and epidermal growth factor and contains L-glutamine and Hepes but no antibiotics.
Order # Product Name Type Unit Price
0411 IMDM SFM Liquid (ready-to-use) 500mL $76.60
datasheet_-_0411_sfm-imdm.pdf
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File Type: pdf
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Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4-6 weeks
pH 7.8 ± 2 (for a 1:1 mixture of Solution A and Solution B)
Osmolality: 270 - 300mOsM

SFM Screening Kit™

The SFM Screening Kit™ is intended for researchers seeking to identify the most appropriate serum-free media formulation for a specific cell type. The kit contains a 100mL sample of each of five serum-free media including proprietary formulations BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™ as well as standard media DMEM/F12, and IMDM. The kit also includes a 25mL sample of FNC Coating Mix® for optimum cell attachment in adherent cell cultures. Each of the media is complete and ready-to-use.
Order # Product Name Type Unit Price
0415 SFM Screening KitTM Liquid (ready-to-use) 1 kit $291.80
applicationmanual_sfmkit_v1-3.pdf
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Kit Components

BRFF-BMZERO™ DMEM / F12 SFM
BRFF-EPM2™ IMDM SFM
BRFF-P4-8F™ FNC Coating Mix®

FNC Coating Mix

FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fiberonectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaESTM offers several different serum-free media that when used in conjunction with FNC Coating Mix®, increase the growth of cell cultures dramatically.
Order # Product Name Type Unit Price
0407 FNC Coating Mix® Liquid (ready-to-use) 100mL $225.30
datasheet_fnc_v2022.pdf
File Size: 253 kb
File Type: pdf
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Product Specifications

Storage Store at 4°C, shipped on blue ice
Stability at 4 ̊C 2 years
pH 7.3 ± 2
Osmolality 270 - 300mOsM

Hepes Buffered Saline

​Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Order # Product Name Type Unit Price
0408 HBS with Phenol Red Liquid (ready-to-use) 500mL $26.06
0409 HBS without Phenol Red Liquid (ready-to-use) 500mL $26.06

Product Specifications

Storage Store at 4°C, supplied frozen
Stability at 4 ̊C 12 - 24 months
pH 7.3 ± 2
Osmolality 270 - 300mOsM

​Freezing Media Pair™

Athena’s Freezing Media Pair™ are a dual-cryopreservation system used to safely store suspensions of viable cells detached from monolayer cultures using the “cold trypsin” method with Athena’s cell dissociation media, PET™. The pair of solutions thoroughly preserve cells which provides superior cell viability upon reconstitution. This Freezing Media Pair™ has been used extensively to cryopreserve a variety of human cell lines grown in serum-free media, including breast and prostate cancer cell lines.
Order # Product Name Type Unit Price
0406 Freezing Media Pair™ Liquid (ready-to-use) 50mL $64.10
datasheet_-_0406_freezing_media_pair_v2018.pdf
File Size: 127 kb
File Type: pdf
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Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C 4-6 weeks
pH 7.8 ± 2 (for a 1:1 mixture of Solution A and Solution B)

Bovine Pituitary Extract

Athena’s Bovine Pituitary Extract is prepared from BSE-free animals. The extracts are prepared from pituitaries harvested from animals in the United States. The pituitaries were obtained only from USDA-inspected facilities where the animals receive pre- and post-mortem inspection and were found to be free of contagious diseases including foot-and-mouth disease, rinderpest, and contagious bovine pleuropneumonia. There are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.
Order # Product Name Type Unit Price
0413 Bovine Pituitary Extract Liquid (ready-to-use) 100mg $228.00
0414 Bovine Pituitary Extract Liquid (ready-to-use) 250mg $498.30

Product Specifications

Storage Store at -80°C, supplied frozen
Stability at 4 ̊C Not Recommended
Protein Concentration 9 - 20mg/mL by Bradford Assay
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