Protein Refolding

AthenaESTM offers a variety of protein refolding reagents that include cyclodextrins, detergents, and specially formulated refolding buffers. Athena’s QuickFoldTM Protein Refolding Kit offers a simple and rapid means of identifying optimal refolding conditions. It employs a comprehensive matrix design, making it easy for researchers to screen a variety of possible refolding conditions quickly.

0600 QuickFold™ Protein Refolding Kit & Buffers

The QuickFold™ Protein Refolding Kit is a screening kit that enables researchers to pinpoint the critical factors for refolding their protein in as little as 1 hour. Unlike many traditional kits, the Quick-Fold™ Kit employs a fractional factorial matrix design that allows the researcher to screen a specific protein in 15 different buffers quickly and easily. Researchers are able to examine a wider range of conditions all in a single experiment, simplifying the process of identifying the best buffer composition and method for the refolding of a given protein. The QuickFold™ Kit comes with enough buffer for 10 refolding experiments, as well as supplemental Dithiothreitol and a Glutathione Redox System. Each buffer is available for individual purchase and is supplemented with the necessary Glutathione Redox System and/or DTT. Individual Buffers come in 500mL and 1L amounts.

Kit Components

Buffers 1 - 15 DTT
Glutathione, reduced Glutathione, oxidized
Order # Product Name Type Unit Price
0600 QuickFoldTM Protein Refolding Kit Liquid (ready-to-use) 1 kit $330.90
techbrief_proteinrefolding_print.pdf
File Size: 149 kb
File Type: pdf
Download File
applicationmanual_quickfold_v2.2.pdf
File Size: 494 kb
File Type: pdf
Download File
proteinrefoldinganalysistemplate.xls
File Size: 16 kb
File Type: xls
Download File

Product Specifications

Individual Refolding Buffers Type 500mL Price 1L Price
QuickFold™ Protein Refolding Buffer 1 Liquid (ready-to-use) 0612-1 $247.60 0613-1 $410.10
QuickFold™ Protein Refolding Buffer 2 Liquid (ready-to-use) 0612-2 $247.60 0613-2 $410.10
QuickFold™ Protein Refolding Buffer 3 Liquid (ready-to-use) 0612-3 $247.60 0613-3 $410.10
QuickFold™ Protein Refolding Buffer 4 Liquid (ready-to-use) 0612-4 $247.60 0613-4 $410.10
QuickFold™ Protein Refolding Buffer 5 Liquid (ready-to-use) 0612-5 $247.60 0613-5 $410.10
QuickFold™ Protein Refolding Buffer 6 Liquid (ready-to-use) 0612-6 $247.60 0613-6 $410.10
QuickFold™ Protein Refolding Buffer 7 Liquid (ready-to-use) 0612-7 $247.60 0613-7 $410.10
QuickFold™ Protein Refolding Buffer 8 Liquid (ready-to-use) 0612-8 $247.60 0613-8 $410.10
QuickFold™ Protein Refolding Buffer 9 Liquid (ready-to-use) 0612-9 $247.60 0613-9 $410.10
QuickFold™ Protein Refolding Buffer 10 Liquid (ready-to-use) 0612-10 $247.60 0613-10 $410.10
QuickFold™ Protein Refolding Buffer 11 Liquid (ready-to-use) 0612-11 $247.60 0613-11 $410.10
QuickFold™ Protein Refolding Buffer 12 Liquid (ready-to-use) 0612-12 $247.60 0613-12 $410.10
QuickFold™ Protein Refolding Buffer 13 Liquid (ready-to-use) 0612-13 $247.60 0613-13 $410.10
QuickFold™ Protein Refolding Buffer 14 Liquid (ready-to-use) 0612-14 $247.60 0613-14 $410.10
QuickFold™ Protein Refolding Buffer 15 Liquid (ready-to-use) 0612-15 $247.60 0613-15 $410.10

0601 Detergent Screening Kit & Individual Detergents

Detergents have been shown to aid in the protein refolding process both of native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100mL quantities.

Kit Components

100 mM Triton-X 100 mM POE(10)L
100 mM Tween 80 100 mM TTAB
100 mM CTAB 100 mM Zwittergent 3-14
Order # Product Name Type Unit Price
0601 Detergent Screening Kit Liquid (ready-to-use) 1 kit $211.50
0603 100 mM Triton-X Liquid (ready-to-use) 100 mL $56.10
0604 100 mM Tween 80 Liquid (ready-to-use) 100 mL $56.10
0605 100 mM CTAB Liquid (ready-to-use) 100 mL $56.10
0606 100 mM POE(10)L 100 mL 1 kit $56.10
0607 100 mM TTAB Liquid (ready-to-use) 100 mL $77.40
0608 100 mM Zwittergent 3-14 Liquid (ready-to-use) 100 mL $359.60

0602 Cyclodextrin Screening Kit & Individual Cyclodextrins

Cyclodextrins’ unique molecular structure greatly promotes protein refolding. Cyclodextrins can suppress protein aggregation, protect against degradation and loss of protein function. They also bind well to detergents (aggregation suppressors), which allows for easy separation of the detergent from the protein, encouraging proper refolding. Different
cyclodextrins act and respond differently to different protein structures. Because of this, AthenaES™ provides 3 of the most common cyclodextrins in a simple screening kit so that the ideal conditions for refolding a given protein can be found quickly and easily. The Cyclodextrin Screening Kit can be particularly useful when paired with the Detergent Screening Kit. Individual cyclodextrins are also available for purchase.

Kit Components

100 mM β-cyclodextrin 50 mM α-cyclodextrin
100 mM methyl-β-cyclodextrin
Order # Product Name Type Unit Price
0602 Cyclodextrin Screening Kit Liquid (ready-to-use) 1 kit $307.50
0609 100 mM β-cyclodextrin Liquid (ready-to-use) 100 mL $180.40
0610 100 mM methyl-β-cyclodextrin Liquid (ready-to-use) 100 mL $1,156.70
0611 50 mM α-cyclodextrin Liquid (ready-to-use) 100 mL $350.51

0201 PDQ™ Protease Assay

Picture
The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease activity in aqueous samples. The proprietary substrate responds to a wide range of proteases including serine, metallo, aspartate and cysteine proteases such as collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. PDQ™ can be used with just a few simple steps to measure protease activity and requires no centrifugation. The substrate is a cross-linked matrix containing protein substrate and a dye-protein conjugate. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity and can detect nanogram quantities. Each kit is supplied with a trypsin solution for generating standard curves (BAEE equivalent units). PDQ™ is supplied in 48 ready-to-use plasict vials. ​

Product Specifications

Storage Store at 4°C
Stability at 4 ̊C 3 months from the date of manufacture
Order # Product Name Type Price
0201 PDQ™ Protease Assay 48 Vials $190.50
© Copyright, 2022 Athena Environmental Sciences, Inc.
Phone: 410-455-6319
Fax: 410-455-1155