Picture
A soft tissue metastasis stained with, from left to right and top to bottom, H&E (A), NKX3.1 (B), BSA (C) and PSAP (D). Note the lack of staining with PSA, while NKX3.1 and PSAP still stain strongly. (Fig. 4 from Gurel et. al., 2010.)

Anti-NKX3.1 Antibody

Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein.  The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1).  The IgG fraction was prepared by Protein A affinity chromatography.  NKX3.1 is located on human chromosome 8p21.21.  It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (2,3).  Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells(5).  Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein.  To improve the specificity of the antibody, only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.
References:
1.  Bethel, C. R. et al. 2006. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia and adenocarcinoma: association with Gleason score and chromosome 8p deletion. Cancer Res. 66:10683-10690.
2.  Abate-Shen, C., Shen, M. M. and Gelmann, E. 2008. Integrating differentiation and cancer: the Nkx3.1 homeobox gene in prostate organogenesis and carcinogenesis. Differentation; research in biological diversity. 76:717-727.
3.  Gurel, B. et al. 2010. NKX3.1 as a marker of prostatic origin in metastatic tumores. Am. J. Surg. Pathol. 34(8):1097-1105.
4.  Guan, B., Pungaltya, P. et al. 2008. Ubiquitination by TOPORS regulates the prostate tumor suppressor NKX3.1. J. Biol. Chem. 283(8):4834-4840
5.  Bieberich, C. J., Fujita, K., He, W. W., and Jay, G. (1996). Prostate-specific and androgen-dependent expression of a novel homeobox gene. J Biol Chem 271, 31779-31782.
Order # Product Name Unit Price
0317 Anti-human NKX3.1 100 μg $385.00
0315 Anti-murine NKX3.1 30 μg Affinity Pure $485.20
0314 Anti-human NKX3.1 1 g Inquire

Product Specifications

Liquid Storage 4 ̊C, Stable for 1 months -20°C, Stable for 1 year
Lyophilized -20°C, Stable for 2 years
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Anti-YebF Antisera

Anti-YebF antisera is used for detecting the YebF protein in immunoassays. The antisera was prepared by injecting rabbits with purified YebF protein isolated from E. coli strain HMS174 harboring pAES40.
​Store product desiccated at -20o C. Store reconstituted product at 4o C
Order # Product Name Type Price
0313 Anti-YebF Antisera 0.5 ml Quotation

I27O™ AFM Reference Protein

I27O™ is a unique recombinant poly-protein used for the calibration of and as a reference for force extension experiments using atomic force microscopes. It is supplied in a ready-to-use solution. The protein is composed of eight repeats of the Ig 27 domain of human titin. Single molecule measurements give up to eight saw-tooth force-extension curves with the specifications below. The protein has durable elasticity that allows for repeated unfolding and refolding.

Product Specifications

Parameter Value
Distance between peaks 24.1 ± 0.34nm
Force Peaks 204 ± 26pN
Persistence Length 0.30 ± 0.07nm
Contour Length (per domain) 28.4 ± 0.3nm
Order # Product Name Type Price
0304 I27O™ AFM Reference Protein Liquid (ready-to-use) 100μg $507.10

​Bacterial Esterases

Cold-tolerant bacterial esterases and lipases have the unusual property of a flexible structure which allows these enzymes to accommodate a broad range of substrates and to be more solvent tolerant. These properties coupled with the stereoselectivity of enzymes, make esterases and lipases ideal catalysts for the chemical modification of pharmacophore molecules. Employing an array of techniques to identify esterase/lipase activity for industrial processes, an in-house microbial collection of psychrophilic microbes was used to screen for specific esterase/lipase activities. Two marine bacteria yielded esterase/lipase genes encoding LypA (V. cholera) and VlpA (arctic-derived Vibro sp.). These enzymes have broad spectrum substrate activity and VlpA exhibits tolerance to solvents by retaining its catalytic activity in buffer-solvent mixtures.

Product Specifications

Enzyme EC Classification Source Special Properties
TesA EC 3.1.1.5 thioesterase from E. coli Mesophilic, Substrates: diacyl glycerides, acyl-thioesters, and r-nitrophenyl esters
LypA EC 3.1.1.5 lysophospholipase L2 from Vibrio cholera Mesophilic, Substrates: diacyl glycerides andr-nitrophenyl esters, poor thioesterase activity
VlpA EC 3.1.1.3 lipase from a marine Vibrio sp. Psychrophilic, Substrates: broad activity with short and long chain acryl esters, solvent tolerant
Order # Product Name Type Price
0305-1 LypA Enzyme Solution 100 Units $435.60
0305-5 LypA Enzyme Solution 500 Units $1,940.40
0306-1 TesA Enzyme Solution 100 Units $435.60
0306-5 TesA Enzyme Solution 500 Units $1,940.40
0307-1 VlpA Enzyme Solution 100 Units $507.10
0307-5 VlpA Enzyme Solution 500 Units $2,262.70

Gaussia Luciferase

​Gaussia luciferase is a luminescent protein with quantum yields higher than the luciferases from Renilla sp. and is up to 1,000 times brighter than firefly luciferase. It is ATP-independent, requiring only coelenterazine and O2 as substrates. Gaussia’s high quantum yields make it particularly useful in immunoassays.

Product Specifications

Protein Analysis Amino Acids % Frequency
Length 185 aa Charged (RKHYCDE) 33.51
Molecular Weight 18,999 Acidic (DE) 12.97
Molar Extinction Coefficient 8,290 Basic (KR) 12.97
Isoelectric Point 6.88 Polar (NCQSTY) 21.08
Charge at pH 7 -0.15 Hydrophobic (AILFWV) 35.68
Order # Product Name Unit Price
0308-1 Gaussia luciferase 2x 1mg Quotation
0308-100 Gaussia luciferase 2x 100μg Quotation

Renilla mullerei Luciferase & Renilla reniformis GFP

Renilla mullerei and Renilla reniformis are species of soft coral made up of colonies of polyps that emit green fluorescence. The green fluorescent protein obtained from this organism exhibits nearly symmetrical excitation and emission peaks. Recombinant Renilla reniformis GFP has the same level of fluorescence as the native protein.

R. mullerei luciferase Specifications

Protein Analysis Amino Acids % Frequency
Length 238 aa Charged (RKHYCDE) 30.67
Molecular Weight 27,120 Acidic (DE) 10.92
Molar Extinction Coefficient 23,970 Basic (KR) 10.08
Isoelectric Point 6.34 Polar (NCQSTY) 27.31
Charge at pH 7 -1.72 Hydrophobic (AILFWV) 31.93

R. reniformis GFP Specifications

Protein Analysis Amino Acids % Frequency
Length 233 aa Charged (RKHYCDE) 30.47
Molecular Weight 25,990 Acidic (DE) 11.59
Molar Extinction Coefficient 15,840 Basic (KR) 10.3
Isoelectric Point 6.19 Polar (NCQSTY) 25.75
Charge at pH 7 -2.61 Hydrophobic (AILFWV) 32.62
Order # Product Name Unit Price
0309-1 Renilla mullerei luciferase 1mg Quotation
0309-100 Renilla mullerei luciferase 100μg Quotation
0310-1 Renilla reniformis Green Fluorescent Protein 1mg Quotation
0310-10 Renilla reniformis Green Fluorescent Protein 10mg Quotation

Ptilosarcus Green Fluorescent Protein

Ptilosarcus gurneyi (orange sea pen, sea feather) is a species of soft coral that emits a strong green fluorescence when disturbed. The green fluorescent protein obtained from this organism exhibits nearly symmetrical excitation and emission peaks. This is in contrast to the more commonly employed green fluorescent protein from Aequorea victoria which has two wavelengths at which excitation occurs and a broader emission spectra.

Product Specifications

Protein Analysis Amino Acids % Frequency
Length 238 aa Charged (RKHYCDE) 31.51
Molecular Weight 27,045 Acidic (DE) 12.18
Molar Extinction Coefficient 22,450 Basic (KR) 10.08
Isoelectric Point 5.92 Polar (NCQSTY) 24.37
Charge at pH 7 -4.52 Hydrophobic (AILFWV) 33.19

Ordering Information

Order # Product Name Unit Price
0311-1 Ptilosarcus Green Fluorescent Protein 1mg Quotation
0311-10 Ptilosarcus Green Fluorescent Protein 10mg Quotation
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