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Expression Media​

Expression Media:   Kits    LB and APF LB Broth™    Turbo and Turbo Prime Broth™    Superior and Superior Prime Broth™
Power and Power Prime Broth™    Hyper Broth™    Glucose M9Y
​
Accessory Expression Products:   Atholate™   Augmedium™ and LB Booster™​   
 

Hyper Broth™

Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena’s Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 5 times that of LB Broth depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is shipped with the necessary Glucose Nutrient Mix.
Order # Product Name Type Price
0107 Hyper Broth™ Powder 500g $51.30
0107S Hyper Broth™ 1L Single-use-Packet (box of 10) $59.00
0118 Hyper Broth™ Liquid (ready-to-use) 500mL $28.20
Picture
(0109 Glucose Nutrient Mix is included with the above products)
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Glucose Nutrient Mix

Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.
Order # Product Name Type Price
0109 Glucose Nutrient Mix Powder 150g $20.30
Picture
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Phone: 410-455-6319
Fax: 410-455-1155

  • Home
  • Products
    • Protein Expression > >
      • Expression Media > >
        • Optimization Kits
        • LB and APF LB Broth
        • Turbo and Turbo Prime Broth
        • Superior and Superior Prime Broth
        • Power and Power Prime Broth
        • Hyper Broth
        • Glucose M9Y
        • Atholate
        • Augmedium ​and LB Booster
      • Protein Refolding
      • Examples
    • Cell Culture > >
      • NEW! Classical Media
      • Serum-free Media > >
        • BRFF-BMZERO
        • BRFF-EPM2
        • BRFF-HPC1
        • ​BRFF-P4-8F
        • DMEM / F12 Serum-Free Medium
        • IMDM Serum-Free Medium
        • SFM Screening Kit
      • Accessory Products > >
        • FNC Coating Mix
        • Freezing Media Pair
        • PET
        • Bovine Pituitary Extract
        • Hepes Buffered Saline
        • Waymouth Cryopreservation Medium
        • L-Alanyl-L-Glutamine
      • Custom Medium Mfg
      • SILAC Media
      • Stem Cells
    • Specialty Proteins
    • Enzyme Assays
    • Manufacturing Services >
      • Biochromatography
  • Support
  • Contact
  • Order Page
    • Distributors