N-acetyl-D-glucosamine Assay

N-acetyl-D-glucosamine (GlcNAc) is phosphorylated by the enzyme GlcNAc Kinase (NagK) and ATP to form GlcNAc-6-phosphate and ADP. Using a coupled reaction in the presence of NADH, the amount of NAD formed is stoichiometric with the amount of GlcNAc, and is measured by a decrease in absorbance at 340 nm.
Calibrator curves of GlcNAc using a kinetic assay performed in a microplate (left panel) or cuvette (right panel).  The assay was performed as per the method described in the applications manual.
Order # Product Name Unit Price
0210 GlcNAc Assay Kit Inquire
Each kit contains enough reagent for 500 microplate assays (0.2 ml each reaction) or 100 cuvette assays (1 mL each reaction).
nagk_assay_manual_v1.pdf
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Patent Pending

PDQ Protease Assay™

The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease activity in aqueous samples. The proprietary substrate responds to a wide range of proteases including serine, metallo, aspartate and cysteine proteases such as collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. PDQ™ can be used with just a few simple steps to measure protease activity and requires no centrifugation. The substrate is a cross-linked matrix containing protein substrate and a dye-protein conjugate. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity and can detect nanogram quantities. Each kit is supplied with a trypsin solution for generating standard curves (BAEE equivalent units). PDQ™ is supplied in 48 ready-to-use plastic vials.

The graph on the right shows the time-course degradation of the PDQ™ Protease Assay substrate by papain, chymotrypsin, proteinase K, and collagenase. Duplicate reactions were incubated at 37°C and 0.2 N NaOH was added to the vials to stop the reaction at the indicated times. The absorbance at 450nm was measured by transferring the reaction mixture to spectrophotometric cuvettes. The results of this experiment show the increased absorption over time due to protelytic degradation of the substrate, and the ability of the assay to detect a wide range of proteases.

Order # Product Name Unit Price
0201 PDQ Protease Assay Kit, 48 Assays $190.50
datasheet-_0201_pdq_v2021_1-page.pdf
File Size: 151 kb
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techbrief_onestepprotease_print.pdf
File Size: 193 kb
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