Expression Media​

Athena's proprietary Protein Expression products are world renowned for their reliability and superior performance for the production of recombinant proteins. They are buffered at different pH values and utilize varying carbon sources.

Many of our products are specifically designed to help solve protein expression problems.  Athena’s Expression Media will help increase the production of your recombinant protein even if you are obtaining good results using traditional LB Broth.  The use of any of Athena’s proprietary formulations will yield higher cell densities, increased plasmid prep concentrations, and higher protein expression levels.  More biomass per unit volume yields more protein which provides for a more cost-effective means of producing your recombinant protein.  In other words, you will likely need less culture volume to recover the same amount of protein or produce more using the same volume as with LB Broth.

0187 Expression Media Selection Kit™ (ready-to-use)

New! The Expression Media Selection Kit contains sterile liquid aliquots of 15 media, including 11 proprietary formulations, reference media, as well as Animal Product Free (APF) versions of Miller and Lennox.  The production of a given recombinant protein can vary significantly depending on the type of medium employed, and the EMSK enables researchers to identify the best media formulation that yields the highest amount of target recombinant protein or plasmid production.

Complete Media Optimization Kit™ (powder packets)

The Complete Media Optimization Kit™ (0185) contains powder packets to make one liter of each media included.  The Complete MOK™ provides 13 expression broths (11 proprietary expression media, Animal Product Free Certified™ LB (Miller) Broth, as well as Glucose M9Y), and reference media LB Broth (Miller).  ​

LB Broth & Animal-Product-Free LB Broth™

​Athena offers three variations of the basic nutrient-rich LB Broth media (Miller, Lennox, and Luria) as well as their APF Certified™ equivalents made using Athena’s proprietary blended plant protein hydrolysate: Atholate™. The formulations each provide an abundance of peptides and peptones, vitamins and trace elements, and differ only in sodium chloride content and whether they were made with casein hydrolysate or Atholate™. The differences allow the researcher to select which formula will provide the ideal osmotic conditions for their particular bacterial strain and desired culture conditions. Each LB Broth and APF LB Broth can be custom ordered in bulk.

Turbo Broth™ & Turbo Prime Broth™

Turbo Broth™, Turbo Prime Broth™ and the new Turbo Prime-olate™ are proprietary media formulations used for the cultivation of recombinant strains of E. coli. Both Turbo Prime™ and Turbo Primeolate™ are APF Certified™. Turbo Prime-olate™ is made with Atholate™, Athena’s proprietary blended plant protein hydrolysate. The media improve the yield of recombinant proteins and typically produce cell yields 4 to 5 times higher than that of LB Broth in shake flask cultures depending on the strain. They have higher plasmid copy numbers than LB, which can enhance expression. Each medium uses glycerol for a carbon source and has a rich nutrient base of amino acids, vitamins, inorganic minerals and trace minerals. The media are buffered at pH 7.2 ± 0.2 with potassium phosphate to prevent a change in pH and provide a source of phosphate. These media are ideal as the base for fermentation cultures. ​

​Superior Broth™ & Superior Prime Broth™

Superior Broth™, Superior Prime Broth™ and new Superior Primeolate™, are proprietary media formulations developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The formulations are complex, supplying a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2 ± 0.2 to prevent acidification of the medium during metabolism of the glucose. Expression of recombinant proteins should be induced at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth. Superior Broth™ shows the highest plasmid copy numbers among the AthenaES™ family of media. This medium is an excellent choice for large-scale plasmid preparations. Biomass yields will depend on the process and the strain employed.

​Power Broth™ & Power Prime Broth™

ower Broth™, Power Prime Broth™, and Power Primeolate™ are proprietary media formulations developed by AthenaES™ for improved expression of recombinant proteins in E. coli. These media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than Terrific Broth. The media are buffered at pH 6.8 ± 0.2. Power Prime™ and Power Primeolate™ are APF Certified™. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power Broth™ and Power Prime Broth™ can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is a favorable base for fermentation cultures. Biomass yields will depend on the process and the strain employed.​

Hyper Broth™

Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena’s Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 5 times that of LB Broth depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is shipped with the necessary Glucose Nutrient Mix.​

Glucose M9Y​

Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena’s Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 5 times that of LB Broth depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is shipped with the necessary Glucose Nutrient Mix.​

Glucose Nutrient Mix

Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.​

Atholate™

Atholate™ is an APF Certified™ proprietary blend of plant protein hydrolysates for use in the preparation of microbiological media. Atholate™ was developed as an Animal-Product-Free alternative to casein hydrolysate in common expression media such as LB Broth. It is designed to outperform standard casein hydrolysate while maintaining the same nutritional characteristics. Athena’s newest expression broths, the Prime-olate™ series, feature Atholate™ as a key component. Atholate™ is available in bulk quantities.

Augmedium™

Augmedium™ is a medium additive which conditions cells prior to induction during a recombinant protein expression protocol. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an inclusion body or as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100mL and 500mL amounts.

LB Booster™

LB Booster™ is a medium additive that increases the level of production of recombinant proteins. Particularly suitable for improving the productivity of LB Broth, LB Booster™ can increase biomass levels by a factor of up to 5 in any medium formulation. In addition, the supplement provides essential nutrients selected for their ability to increase the relative level of recombinant protein accumulation. LB Booster™ is supplied as a powder and as a ready-to-use 20x concentrate. LB Booster™ is a proprietary blend of fructose and nutrient supplements.
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