Expression Media​

Athena's proprietary Protein Expression products are world renowned for their reliability and superior performance for the production of recombinant proteins.  Many of our products are specifically designed to help solve protein expression problems.  Athena’s Expression Media will help increase the production of your recombinant protein even if you are obtaining good results using traditional LB Broth.  The use of any of Athena’s proprietary formulations will yield higher cell densities.  More biomass per unit volume yields more protein which provides for a more cost-effective means of producing your recombinant protein.  In other words, you will likely need less culture volume to recover the same amount of protein or produce more using the same volume as with LB Broth.
selectionofexpressionmediatechnical.pdf
File Size: 406 kb
File Type: pdf
Download File

Complete Media Optimization Kit™​

New!  The Complete Media Optimization Kit™ contains all 10 of Athena's Expression Media formulations along with 3 reference media.  Since the production of a given recombinant protein can vary significantly depending on the type of medium employed, the MOK™'s provide 11 proprietary expression broths (10 Expression Media plus Animal Produce Free Certified™ LB (Miller) Broth), as well as Glucose M9Y, and LB Broth (Miller).  The Complete MOK™ combines the 3 original MOK's into one kit, giving researchers the added convenience of being able to screen all 10 Expression Media formulations.  The MOK™ enables researchers to identify the best media formulation that yields the highest amount of target recombinant protein.  Each dry powder kit contains enough mix to prepare 1 liter of each of the 13 media.

Kit Components

Cat. No. Medium Cat. No. Medium
0103 LB (Miller) Broth 0133 APF LB (Miller) Broth
0104 Turbo Broth™ 0110 Turbo Prime™Broth
0105 Superior Broth™ 0111 Superior Prime Broth™
0106 Power Broth™ 0112 Power Prime Broth™
0107 Hyper Broth™ 0108 Glucose M9Y
0160 Turbo Prime-olate Broth™ 0161 Superior Prime-olate Broth™
0162 Power Prime-olate Broth™ 0109 Glucose Nutrient Mix
applicationmanual_mok-v3.pdf
File Size: 1251 kb
File Type: pdf
Download File
selectionofexpressionmediatechnical.pdf
File Size: 406 kb
File Type: pdf
Download File
Order # Product Name Type Price
0185 Complete Media Optimization Kit™ 1 Kit $153.20
0185-L Complete Media Optimization Kit™ Liquid 1 Kit Inquire

​PERK™: Protein Expression Rescue Kit

The PERK™ (Protein Expression Rescue Kit) was designed to provide the researcher with a tool for determining the best available culture conditions for the production of recombinant proteins in Escherichia coli.  Experience has shown that the accumulation of a given protein in the cytoplasm is dependent upon several factors.  These include the host strain, the composition of the medium and the incubation conditions used.  In the course of optimizing the production of recombinant proteins for clients, Athena’s scientists have developed an algorithm that was specifically designed to maximize the accumulation of recombinant proteins expressed in E. coli.  Figure 1 depicts the experimental flow chart followed for this algorithm.
Picture
Figure 1. Algorithm for optimizing the production of recombinant proteins.
applicationmanual_perk_v3.pdf
File Size: 350 kb
File Type: pdf
Download File

Kit Components

Cat. No. Medium Cat. No. Medium
0103 LB (Miller) Broth 0133 APF LB (Miller) Broth
0104 Turbo Broth™ 0110 Turbo Prime™Broth
0105 Superior Broth™ 0111 Superior Prime Broth™
0106 Power Broth™ 0112 Power Prime Broth™
0107 Hyper Broth™ 0108 Glucose M9Y
0160 Turbo Prime-olate Broth™ 0161 Superior Prime-olate Broth™
0162 Power Prime-olate Broth™ 0109 Glucose Nutrient Mix
0125 LB*Booster™ 0123 Augmedium™
Order # Product Name Type Price
0186 PERK™ Protein Expression Rescue Kit 1 Kit $213.80
0186-L PERK™ Protein Expression Rescue Kit, Liquid 1 Kit Inquire

LB Broth & Animal-Product-Free LB Broth™

​Athena offers three variations of the basic nutrient-rich LB Broth media (Miller, Lennox, and Luria) as well as their APF Certified™ equivalents made using Athena’s proprietary blended plant protein hydrolysate: Atholate™. The formulations each provide an abundance of peptides and peptones, vitamins and trace elements, and differ only in sodium chloride content and whether they were made with casein hydrolysate or Atholate™. The differences allow the researcher to select which formula will provide the ideal osmotic conditions for their particular bacterial strain and desired culture conditions. Each LB Broth and APF LB Broth can be custom ordered in bulk.
techbrief_apflbbroth_print.pdf
File Size: 325 kb
File Type: pdf
Download File
Order # Product Name Type Price
0101 LB Broth (Luria) Powder 500g $64.50
0102 LB Broth (Lennox) Powder 500g $56.50
0113 LB Broth (Lennox) Liquid (ready-to-use) 500mL $26.20
0103 LB Broth (Miller) Powder 500g $49.80
0130 LB Broth (Miller) Powder 1L Single-use-Packets (Box of 10) $60.50
0114 LB Broth (Miller) Liquid (ready-to-use) 500mL $26.20
0131 APF LB Broth (Luria) Powder 500g $129.20
0131-S APF LB Broth (Luria) Powder 1L Single-use-Packets (Box of 10) $134.30
0174 APF LB Broth (Luria) Liquid (ready-to-use) 500mL $28.80
0132 APF LB Broth (Lennox) Powder 500g $92.40
0132-S APF LB Broth (Lennox) Powder 1L Single-use-Packets (Box of 10) $98.00
0175 APF LB Broth (Lennox) Liquid (ready-to-use) 500mL $28.80
0133 APF LB Broth (Miller) Powder 500g $92.40
0133-S APF LB Broth (Miller) Powder 1L Single-use-Packets (Box of 10) $98.00
0173 APF LB Broth (Miller) Liquid (ready-to-use) 500mL $28.80

Turbo Broth™ & Turbo Prime Broth™

Turbo Broth™, Turbo Prime Broth™ and the new Turbo Prime-olate™ are proprietary media formulations used for the cultivation of recombinant strains of E. coli. Both Turbo Prime™ and Turbo Primeolate™ are APF Certified™. Turbo Prime-olate™ is made with Atholate™, Athena’s proprietary blended plant protein hydrolysate. The media improve the yield of recombinant proteins and typically produce cell yields 4 to 5 times higher than that of LB Broth in shake flask cultures depending on the strain. They have higher plasmid copy numbers than LB, which can enhance expression. Each medium uses glycerol for a carbon source and has a rich nutrient base of amino acids, vitamins, inorganic minerals and trace minerals. The media are buffered at pH 7.2 ± 0.2 with potassium phosphate to prevent a change in pH and provide a source of phosphate. These media are ideal as the base for fermentation cultures. ​
Order # Product Name Type Price
0104 Turbo Broth™ Powder 500g $65.90
0104S Turbo Broth™ 1L Single-use-Packet (box of 10) $73.40
0115 Turbo Broth™ Liquid (ready-to-use) 500mL $26.20
0110 Turbo Prime Broth™ 500g $67.90
0110S Turbo Prime Broth™ Powder 1L Single-use-Packet (box of 10) $75.90
0120 Turbo Prime Broth™ Liquid (ready-to-use) 500mL $29.40
0160 Turbo Prime-olate™ 500g $71.60
0160S Turbo Prime-olate™ Powder 1L Single-use-Packets (Box of 10) $71.60
0170 Turbo Prime-olate™ Liquid (ready-to-use) 500mL $28.80

​Superior Broth™ & Superior Prime Broth™

Superior Broth™, Superior Prime Broth™ and new Superior Primeolate™, are proprietary media formulations developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The formulations are complex, supplying a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2 ± 0.2 to prevent acidification of the medium during metabolism of the glucose. Expression of recombinant proteins should be induced at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth. Superior Broth™ shows the highest plasmid copy numbers among the AthenaES™ family of media. This medium is an excellent choice for large-scale plasmid preparations. Biomass yields will depend on the process and the strain employed.
Order # Product Name Type Price
0105 Superior Broth™ Powder 500g $69.00
0105S Superior Broth™ 1L Single-use-Packet (box of 10) $79.00
0116 Superior Broth™ Liquid (ready-to-use) 500mL $26.20
0111 Superior Prime Broth™ 500g $70.20
0111S Superior Prime Broth™ Powder 1L Single-use-Packet (box of 10) $78.50
0121 Superior Prime Broth™ Liquid (ready-to-use) 500mL $29.20
0161 Superior Prime-olate™ Powder 500g $81.40
0161S Superior Prime-olate™ Powder 1L Single-use-Packet (box of 10) $81.40
0171 Superior Prime-olate™ Liquid (ready-to-use) 500mL $28.80

Power Broth™ & Power Prime Broth™

Power BrothTM, Power Prime BrothTM, and Power Primeolate™ are proprietary media formulations developed by AthenaESTM for improved expression of recombinant proteins in E. coli. These media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than Terrific Broth. The media are buffered at pH 6.8 ± 0.2. Power PrimeTM and Power Primeolate™ are APF CertifiedTM. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power BrothTM and Power Prime BrothTM can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is a favorable base for fermentation cultures. Biomass yields will depend on the process and the strain employed.
Order # Product Name Type Price
0106 Power Broth™ Powder 500g $56.20
0106S Power Broth™ 1L Single-use-Packet (box of 10) $63.30
0117 Power Broth™ Liquid (ready-to-use) 500mL $26.20
0112 Power Prime Broth™ Powder 500g $69.30
0112S Power Prime Broth™ Powder 1L Single-use-Packet (box of 10) $82.00
0122 Power Prime Broth™ Liquid (ready-to-use) 500mL $29.40
0162 Power Prime-olate™ Powder 500g $81.40
0162S Power Prime-olate™ Powder 1L Single-use-Packet (box of 10) $81.40
0172 Power Prime-olate™ Liquid (ready-to-use) 500mL $28.80

Hyper Broth™

Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena’s Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 5 times that of LB Broth depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is shipped with the necessary Glucose Nutrient Mix.
Order # Product Name Type Price
0107 Hyper Broth™ Powder 500g $49.80
0107S Hyper Broth™ 1L Single-use-Packet (box of 10) $57.30
0118 Hyper Broth™ Liquid (ready-to-use) 500mL $26.20
(Glucose Nutrient Mix is included with the above products)

Glucose M9Y

Glucose M9Y is an APF Certified™ minimal media formulation supplemented with yeast extract for the cultivation of E. coli. It is based on M9 salts with a pH of 6.9 ± 0.2 and employs glucose as the carbon source1. Buffering is provided by a sodium-potassium phosphate system. Ammonium chloride provides a nitrogen source. Yeast extract is added to improve the protein production capacity of the medium and to permit cultivation of auxotrophic strains by supplying a source of amino acids, vitamins and minerals. Our formulation differs from the traditional formulation of Glucose M9, which employs casamino acids. Our experience has shown that yeast extract typically gives higher yields of recombinant proteins than casamino acids. Glucose M9Y powder is supplied with a separate Glucose Nutrient Mix.
Order # Product Name Type Price
0108 Glucose M9Y Powder 500g $39.60
0108S Glucose M9Y 1L Single-use-Packet (box of 10) $44.30
0119 Glucose M9Y Liquid (ready-to-use) 500mL $26.20
(0109 Glucose Nutrient Mix is included with the above products)

Glucose Nutrient Mix

Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.
Order # Product Name Type Price
0109 Glucose Nutrient Mix Powder 150g $20.30
(Glucose Nutrient Mix is included with Hyper Broth™ [0107/0118] and Glucose M9Y [0108/0119])​

Atholate™

Atholate™ is an APF Certified™ proprietary blend of plant protein hydrolysates for use in the preparation of microbiological media. Atholate™ was developed as an Animal-Product-Free alternative to casein hydrolysate in common expression media such as LB Broth. It is designed to outperform standard casein hydrolysate while maintaining the same nutritional characteristics. Athena’s newest expression broths, the Prime-olate™ series, feature Atholate™ as a key component. Atholate™ is available in bulk quantities.
Order # Product Name Type Price
0134 Atholate™ Powder 500g $160.60
0134-1 Atholate™ Powder 1kg $305.50
0134-2.5 Atholate™ Powder 2.5kg $704.50
0134-10 Atholate™ Powder 10kg $2,437.00

LB Booster™

LB Booster™ is a medium additive that increases the level of production of recombinant proteins. Particularly suitable for improving the productivity of LB Broth, LB Booster™ can increase biomass levels by a factor of up to 5 in any medium formulation. In addition, the supplement provides essential nutrients selected for their ability to increase the relative level of recombinant protein accumulation. LB Booster™ is supplied as a powder and as a ready-to-use 20x concentrate. LB Booster™ is a proprietary blend of fructose and nutrient supplements.
Order # Product Name Type Price
0125 LB Booster™ Powder for 100mL stock 1 packet $42.90
0126 LB Booster™ Powder for 500mL stock 1 packet $163.90
0127 LB Booster™ 20x Liquid (ready-to-use) 500mL $202.40

Augmedium™ ​

Augmedium™ is a medium additive which conditions cells prior to induction during a recombinant protein expression protocol. This pre-induction conditioner increases the level of chaperone proteins which can improve the fraction of product that accumulates as soluble protein. Augmedium™ is specifically intended for use with strains in which the target protein accumulates as an inclusion body or as an insoluble aggregate. Augmedium™ is supplied as a powder for preparation of 50x concentrated stock solutions. It is available in 100mL and 500mL amounts.
Order # Product Name Type Price
0123 Augmedium™ Powder 100mL Stock $145.00
0124 Augmedium™ Powder 500mL Stock $605.00

​ExpressMax™ Fermentation Media and Kit

Athena's ExpressMax™ series are fermentation media for the production of recombinant proteins. The basal salts are inorganic nutrients optimized to give the highest possible growth rate of E. coli and production of recombinant proteins. The screening kit is intended to aid in formulation development by supplying the basal salts along with the carbon (glucose) and nitrogen sources (yeast extract, soy protein hydrolysate and ) which can be combined in different variations to identify the composition that gives the highest production of the target protein. ExpressMax™ medium is also available pre-formulated for ease of use. These formulations have been designed by Athena's scientists for maximum production of recombinant proteins.
Order # Product Name Type Price
0136 Express Max™ Screening Kit 1 Kit $78.54
0137 Express Max™ Formula 1 500g $72.20
0138 Express Max™ Formula 2 500g $72.20
0139 Express Max™ Formula 3 500g $72.20
0140 Express Max™ Formula 4 500g $72.20
0141 Express Max™ Formula 5 500g $72.20
0142 Express Max™ Formula 6 500g $72.20
0143 Express Max™ Formula 7 500g $72.20
0144 Express Max™ Formula 8 500g $72.20
0145 Express Max™ Basal Salts 500g $40.70
0146 Yeast Extract 500g $59.40
0147 Soy Protein Hydrolysate 500g $59.40
0109 Glucose Nutrient Mix 150g $20.30
0134 Atholate™ 500g $160.6
© Copyright, 2022 Athena Environmental Sciences, Inc.
Phone: 410-455-6319
Fax: 410-455-1155