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Power Broth™, Power Prime Broth™, and Power Primeolate™ are proprietary media formulations developed by AthenaES™ for improved expression of recombinant proteins in E. coli. These media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than Terrific Broth. The media are buffered at pH 6.8 ± 0.2. Power Prime™ and Power Primeolate™ are APF Certified™. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power Broth™ and Power Prime Broth™ can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is a favorable base for fermentation cultures. Biomass yields will depend on the process and the strain employed.
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