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0407 and 0407H FNC Coating Mix®FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES® offers several different serum-free media that when used in conjunction with FNC Coating Mix®, increase the growth of cell cultures dramatically.
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0406 Freezing Media Pair™Athena’s Freezing Media Pair™ are a dual-cryopreservation system used to safely store suspensions of viable cells detached from monolayer cultures using the “cold trypsin” method with Athena’s cell dissociation media, PET™. The pair of solutions thoroughly preserve cells which provides superior cell viability upon reconstitution. This Freezing Media Pair™ has been used extensively to cryopreserve a variety of human cell lines grown in serum-free media, including breast and prostate cancer cell lines.
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0405 PET™PET™ is a superior cell dissociation reagent specially formulated for use under “Cold Trypsin” conditions. The unique blend of polyvinylpyrrolidone, EGTA, and trypsin in a HBS base allows the trypsin to retain enzymatic activity under cool temperatures, unlike standard trypsin which requires a temperature of 37°C. When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent yields more viable cells from monolayers grown in serum-free medium than traditional trypsin formulas.
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0413/0414 Bovine Pituitary ExtractAthena’s Bovine Pituitary Extract is prepared from BSE-free animals. The extracts are prepared from pituitaries harvested from animals in the United States. The pituitaries were obtained only from USDA-inspected facilities where the animals receive pre- and post-mortem inspection and were found to be free of contagious diseases including foot-and-mouth disease, rinderpest, and contagious bovine pleuropneumonia. There are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.
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Hepes Buffered SalineHepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
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Waymouth Cryopreservation MediumWaymouth Cryopreserve Medium is a serum-free ready-to-use reagent for the cryogenic preservation of mammalian cells. The sterile solution is composed of a Minimial Essential Medium (MEM) based with methylcellulose and DMSO as preservation agents. Cells which have been cryopreserved in this medium have been shown to have good viability upon reconstitution.
Composition: 0.087% Methylcellulose, 8.7% DMSO in MEM medium. |
L-Alanyl-L-Glutamine (aka "GlutaMax")L-Alanyl-L-glutamine is a direct replacement for L-glutamine in cell culture media. The alanyl derivative of glutamine is thermally stable, which extends the shelf-life of cell culture medium by limiting deamination of the terminal amino group of the side-chain.
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