Serum-free Media

Athena’s proprietary Serum-Free Media (SFM) have simplified and defined compositions that are developed for use in mammalian tissue culture research -- including drug discovery, physiological, and gene expression studies. Athena provides an array of serum-free media in addition to several supplementary products to aid in the growth and preservation of mammalian cell lines. Viral, bacterial, and fungal contamination of serum is common and presents serious safety hazards for manufacturers of biopharmaceuticals. The use of serum-free media significantly reduces the possibility of this potential contamination.

BRFF-BMZERO (0401)     BRFF-EPM2 (0402)     BRFF-HPC1 (0403)
BRFF-P4-8F (0404)     DMEM / F12 Serum Free (0410)
IMDM Serum Free (0411)     ​SFM Screening Kit (0415)
Accessory Products

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BRFF-BMZERO™ (0401)

​BRFF-BMZERO™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing human breast cell lines. BMZERO™ was developed and has been extensively used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

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BRFF-EPM2™ (0402)

BRFF-EPM2™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors optimized for culturing human esophageal epithelial cell lines. EPM2™ has been used for culturing epithelial cells from explant cultures of human skin as well as for culturing a number of human cancer cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

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BRFF-HPC1™ (0403)

BRFF-HPC-1™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements, growth factors and dihydrotestosterone, designed for establishing new cell lines from human prostate tissue. HPC-1™ was developed and has been extensively used for establishing and maintaining epithelial cell lines from both benign prostatic hyperplasia and prostatic carcinoma. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

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BRFF-P4-8F™ (0404)

BRFF-P4-8F™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing immortalized normal prostatic cell lines such as 267-B1.1 P4-8F™ supports the growth of certain established human prostatic cancer cell lines such as PC-3. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

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DMEM / F12 Serum Free (0410)

DMEM/F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham’s F12 media supplied complete and ready to use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types. DMEM/F12 does not contain phenol red or antibiotics.

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IMDM Serum Free (0411)

Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium. It is supplemented with bovine pituitary extract and epidermal growth factor and contains L-glutamine and Hepes but no antibiotics.

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SFM Screening Kit (0415)

The SFM Screening Kit™ is intended for researchers seeking to identify the most appropriate serum-free media formulation for a specific cell type. The kit contains a 100mL sample of each of five serum-free media including proprietary formulations BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™ as well as standard media DMEM/F12, and IMDM. The kit also includes a 25mL sample of FNC Coating Mix® for optimum cell attachment in adherent cell cultures. Each of the media is complete and ready-to-use.