Cell Culture >

New! Classical Media Formulations (0460-0469)

Designed for the optimal growth and maintenance of cells, these media provide essential nutrients, growth factors, and salts necessary for cell metabolism and function. Athena specializes in small batch custom productions, from standard formulations to highly complex specialty media for a wide variety of cell types. Ask us about tailoring formulations to suit specific nutritional needs!

0460 DMEM High Glucose
0461 DMEM Low Glucose
0462 RPMI 1640

0463 DMEM/F12, basal media
0464 MEM with L-glutamine
0465 Ham's F12

0466 McCoy's 5A
0468 Neural Basal
0469-50 L-Glutamine, 200mM

0470 L-15 Leibovitz, powder
0471 L-15 Leibovitz, liquid

BRFF-BMZERO™ (0401)

BRFF-BMZERO™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing human breast cell lines. BMZERO™ was developed and has been extensively used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

BRFF-EPM2™ (0402)

BRFF-EPM2™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors optimized for culturing human esophageal epithelial cell lines. EPM2™ has been used for culturing epithelial cells from explant cultures of human skin as well as for culturing a number of human cancer cell lines. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

BRFF-HPC1™ (0403)

BRFF-HPC-1™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements, growth factors and dihydrotestosterone, designed for establishing new cell lines from human prostate tissue. HPC-1™ was developed and has been extensively used for establishing and maintaining epithelial cell lines from both benign prostatic hyperplasia and prostatic carcinoma. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

BRFF-P4-8F™ (0404)

BRFF-P4-8F™ is a complete serum-free medium comprised of essential amino acids, vitamins, inorganic salts, trace elements and growth factors designed for culturing immortalized normal prostatic cell lines such as 267-B1.1 P4-8F™ supports the growth of certain established human prostatic cancer cell lines such as PC-3. Optimal attached monolayer cell growth can be achieved when used in conjunction with FNC Coating Mix®.

DMEM / F12 Serum Free (0410)

IMDM Serum Free (0411)

Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium. It is supplemented with bovine pituitary extract and epidermal growth factor and contains L-glutamine and Hepes but no antibiotics.

SFM Screening Kit (0415)

FNC Coating Mix® (0407 and 0407H)

FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES® offers several different serum-free media that when used in conjunction with FNC Coating Mix®, increase the growth of cell cultures dramatically.

Freezing Media Pair™ (0406)

Athena’s Freezing Media Pair™ are a dual-cryopreservation system used to safely store suspensions of viable cells detached from monolayer cultures using the “cold trypsin” method with Athena’s cell dissociation media, PET™. The pair of solutions thoroughly preserve cells which provides superior cell viability upon reconstitution. This Freezing Media Pair™ has been used extensively to cryopreserve a variety of human cell lines grown in serum-free media, including breast and prostate cancer cell lines.

PET™ (0405)

PET™ is a superior cell dissociation reagent specially formulated for use under “Cold Trypsin” conditions. The unique blend of polyvinylpyrrolidone, EGTA, and trypsin in a HBS base allows the trypsin to retain enzymatic activity under cool temperatures, unlike standard trypsin which requires a temperature of 37°C. When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent yields more viable cells from monolayers grown in serum-free medium than traditional trypsin formulas.

Bovine Pituitary Extract (0413 and 0414)

Athena’s Bovine Pituitary Extract is prepared from BSE-free animals. The extracts are prepared from pituitaries harvested from animals in the United States. The pituitaries were obtained only from USDA-inspected facilities where the animals receive pre- and post-mortem inspection and were found to be free of contagious diseases including foot-and-mouth disease, rinderpest, and contagious bovine pleuropneumonia. There are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.

Hepes Buffered Saline (0408 and 0409)

Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.

Waymouth Cryopreservation Medium (0459)

Waymouth Cryopreserve Medium is a serum-free ready-to-use reagent for the cryogenic preservation of mammalian cells. The sterile solution is composed of a Minimial Essential Medium (MEM) based with methylcellulose and DMSO as preservation agents. Cells which have been cryopreserved in this medium have been shown to have good viability upon reconstitution. Composition: 0.087% Methylcellulose, 8.7% DMSO in MEM medium.

L-Alanyl-L-Glutamine (aka "GlutaMax") (0453)

L-Alanyl-L-glutamine is a direct replacement for L-glutamine in cell culture media. The alanyl derivative of glutamine is thermally stable, which extends the shelf-life of cell culture medium by limiting deamination of the terminal amino group of the side-chain.

Custom Manufacturing

Custom Tissue Culture Media AthenaES™ offers custom cell culture media from the standard formulations to the highly complex specialty media for all research needs. Over 250 different custom formulations have been produced including mammalian, plant, insect and bacterial cells for use in drug screening, process development, SILAC (stable culture isotope labeling with amino acids) experiments and animal-product-free applications. We can tailor the media formulation to your specifications.

SILAC Media (0420-0433)

SILAC media is prepared specifically for labeling experiments involving the use of stable amino acid isotopes. Athena provides several of the most commonly used media without L-arginine, L-lysine, L-leucine and L-methionine, the four mostly commonly used heavy isotope labeled amino acids.