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AthenaES™ offers a variety of protein refolding reagents that include cyclodextrins, detergents, and specially formulated refolding buffers. Athena’s QuickFold™ Protein Refolding Kit offers a simple and rapid means of identifying optimal refolding conditions. It employs a comprehensive matrix design, making it easy for researchers to screen a variety of possible refolding conditions quickly.
0600 QuickFold™ Protein Refolding Kit & Buffers
The QuickFold™ Protein Refolding Kit is a screening kit that enables researchers to pinpoint the critical factors for refolding their protein in as little as 1 hour. Unlike many traditional kits, the Quick-Fold™ Kit employs a fractional factorial matrix design that allows the researcher to screen a specific protein in 15 different buffers quickly and easily. Researchers are able to examine a wider range of conditions all in a single experiment, simplifying the process of identifying the best buffer composition and method for the refolding of a given protein. The QuickFold™ Kit comes with enough buffer for 10 refolding experiments, as well as supplemental Dithiothreitol and a Glutathione Redox System. Each buffer is available for individual purchase and is supplemented with the necessary Glutathione Redox System and/or DTT. Individual Buffers come in 500mL and 1L amounts.
Kit Components
| Buffers 1-15 | DTT | Glutathione, reduced | Glutathione, oxidized |
| Order # | Product Name | Type | Unit | Price |
|---|---|---|---|---|
| 0600 | QuickFoldTM Protein Refolding Kit | Liquid (ready-to-use) | 1 kit | $330.90 |
Product Specifications
| Individual Refolding Buffers | Type | 500mL | Price | 1L | Price |
|---|---|---|---|---|---|
| QuickFold™ Protein Refolding Buffer 1 | Liquid (ready-to-use) | 0612-1 | $247.60 | 0613-1 | $410.10 |
| QuickFold™ Protein Refolding Buffer 2 | Liquid (ready-to-use) | 0612-2 | $247.60 | 0613-2 | $410.10 |
| QuickFold™ Protein Refolding Buffer 3 | Liquid (ready-to-use) | 0612-3 | $247.60 | 0613-3 | $410.10 |
| QuickFold™ Protein Refolding Buffer 4 | Liquid (ready-to-use) | 0612-4 | $247.60 | 0613-4 | $410.10 |
| QuickFold™ Protein Refolding Buffer 5 | Liquid (ready-to-use) | 0612-5 | $247.60 | 0613-5 | $410.10 |
| QuickFold™ Protein Refolding Buffer 6 | Liquid (ready-to-use) | 0612-6 | $247.60 | 0613-6 | $410.10 |
| QuickFold™ Protein Refolding Buffer 7 | Liquid (ready-to-use) | 0612-7 | $247.60 | 0613-7 | $410.10 |
| QuickFold™ Protein Refolding Buffer 8 | Liquid (ready-to-use) | 0612-8 | $247.60 | 0613-8 | $410.10 |
| QuickFold™ Protein Refolding Buffer 9 | Liquid (ready-to-use) | 0612-9 | $247.60 | 0613-9 | $410.10 |
| QuickFold™ Protein Refolding Buffer 10 | Liquid (ready-to-use) | 0612-10 | $247.60 | 0613-10 | $410.10 |
| QuickFold™ Protein Refolding Buffer 11 | Liquid (ready-to-use) | 0612-11 | $247.60 | 0613-11 | $410.10 |
| QuickFold™ Protein Refolding Buffer 12 | Liquid (ready-to-use) | 0612-12 | $247.60 | 0613-12 | $410.10 |
| QuickFold™ Protein Refolding Buffer 13 | Liquid (ready-to-use) | 0612-13 | $247.60 | 0613-13 | $410.10 |
| QuickFold™ Protein Refolding Buffer 14 | Liquid (ready-to-use) | 0612-14 | $247.60 | 0613-14 | $410.10 |
| QuickFold™ Protein Refolding Buffer 15 | Liquid (ready-to-use) | 0612-15 | $247.60 | 0613-15 | $410.10 |
0601 Detergent Screening Kit & Individual Detergents
Detergents have been shown to aid in the protein refolding process of both native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex, which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10 mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100 mL quantities.
Kit Components
| 100 mM Triton-X | 100 mM POE(10)L | 100 mM Tween 80 | 100 mM TTAB | 100 mM CTAB | 100 mM Zwittergent 3-14 |
| Order # | Product Name | Type | Unit | Price |
|---|---|---|---|---|
| 0601 | Detergent Screening Kit | Liquid (ready-to-use) | 1 kit | $211.50 |
| 0603 | 100 mM Triton-X | Liquid (ready-to-use) | 100 mL | $56.10 |
| 0604 | 100 mM Tween 80 | Liquid (ready-to-use) | 100 mL | $56.10 |
| 0605 | 100 mM CTAB | Liquid (ready-to-use) | 100 mL | $56.10 |
| 0606 | 100 mM POE(10)L | 100 mL | 1 kit | $56.10 |
| 0607 | 100 mM TTAB | Liquid (ready-to-use) | 100 mL | $77.40 |
| 0608 | 100 mM Zwittergent 3-14 | Liquid (ready-to-use) | 100 mL | $359.60 |
0602 Cyclodextrin Screening Kit & Individual Cyclodextrins
Cyclodextrins’ unique molecular structure greatly promotes protein refolding. Cyclodextrins can suppress protein aggregation and protect against degradation and loss of protein function. They also bind well to detergents (aggregation suppressors), which allows for easy separation of the detergent from the protein, encouraging proper refolding.
Find Your Perfect Match
Different cyclodextrins act and respond differently to different protein structures. Because of this, AthenaES™ provides 3 of the most common cyclodextrins in a simple screening kit so that the ideal conditions for refolding a given protein can be found quickly and easily. The Cyclodextrin Screening Kit can be particularly useful when paired with the Detergent Screening Kit. Individual cyclodextrins are also available for purchase.
Kit Components
| 100 mM β-cyclodextrin | 50 mM α-cyclodextrin | 100 mM methyl-β-cyclodextrin |
| Order # | Product Name | Type | Unit | Price |
|---|---|---|---|---|
| 0602 | Cyclodextrin Screening Kit | Liquid (ready-to-use) | 1 kit | $307.50 |
| 0609 | 100 mM β-cyclodextrin | Liquid (ready-to-use) | 100 mL | $180.40 |
| 0610 | 100 mM methyl-β-cyclodextrin | Liquid (ready-to-use) | 100 mL | $1,156.70 |
| 0611 | 50 mM α-cyclodextrin | Liquid (ready-to-use) | 100 mL | $350.51 |
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