PET

​PET™ is a superior cell dissociation reagent specially formulated for use under “cold trypsin” conditions. The unique blend of polyvinylpyrrolidone, EGTA, and trypsin in an HBS base allows the trypsin to retain enzymatic activity under cool temperatures, unlike standard trypsin, which requires a temperature of 37 °C. When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent yields more viable cells from monolayers grown in serum-free medium than traditional trypsin formulas.