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Optimized Minimal Media
Glucose M9Y is an APF Certified™ minimal media formulation supplemented with yeast extract for the cultivation of E. coli. It is based on M9 salts with a pH of 6.9 ± 0.2 and employs glucose as the carbon source. Buffering is provided by a sodium-potassium phosphate system. Ammonium chloride provides a nitrogen source. Yeast extract is added to improve the protein production capacity of the medium and to permit cultivation of auxotrophic strains by supplying a source of amino acids, vitamins, and minerals.
Superior Yields Over Traditional Formulas
Our formulation differs from the traditional formulation of Glucose M9, which employs casamino acids. Our experience has shown that yeast extract typically gives higher yields of recombinant proteins than casamino acids. Glucose M9Y powder is supplied with a separate glucose nutrient mix.
| Order # | Product Name | Type | Price |
|---|---|---|---|
| 0108 | Glucose M9Y | Powder 500g | $40.70 |
| 0108S | Glucose M9Y | 1L Single-use-Packet (box of 10) | $58.00 |
| 0119 | Glucose M9Y | Liquid (ready-to-use) 500mL | $28.20 |
(0109 Glucose Nutrient Mix is included with the above products)
Glucose Nutrient Mix
Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.
| Order # | Product Name | Type | Price |
|---|---|---|---|
| 0109 | Glucose Nutrient Mix | Powder 150g | $20.30 |
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