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Achieve Maximum Expression
Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation that supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena’s Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.
Nutrient Delivery for Fermentation Success
Cell yields are typically 5 times that of LB broth, depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is shipped with the necessary Glucose Nutrient Mix.
| Order # | Product Name | Type | Price |
|---|---|---|---|
| 0107 | Hyper Broth™ | Powder 500g | $51.30 |
| 0107S | Hyper Broth™ | 1L Single-use-Packet (box of 10) | $59.00 |
| 0118 | Hyper Broth™ | Liquid (ready-to-use) 500mL | $28.20 |
(0109 Glucose Nutrient Mix is included with the above products)
Glucose Nutrient Mix
Glucose Nutrient Mix is an APF Certified™ medium supplement used for enhancing the expression of proteins when using Athena’s Hyper Broth™ or Glucose M9Y. The powder blend consists of glucose as a carbon source and is Glucose Nutrient Mix Powder 150g 0109. Free Available in dry-powder packets. supplemented with calcium chloride and magnesium sulfate. Glucose Nutrient Mix is included with both media, but can now be purchased separately in individual packets.
| Order # | Product Name | Type | Price |
|---|---|---|---|
| 0109 | Glucose Nutrient Mix | Powder 150g | $20.30 |
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