Protein Refolding

AthenaESTM offers a variety of protein refolding reagents that include cyclodextrins, detergents, and specially formulated refolding buffers. Athena’s QuickFoldTM Protein Refolding Kit offers a simple and rapid means of identifying optimal refolding conditions. It employs a comprehensive matrix design, making it easy for researchers to screen a variety of possible refolding conditions quickly.

QuickFold™ Protein Refolding Kit & Buffers

The QuickFoldTM Protein Refolding Kit is a screening kit that enables researchers to pinpoint the critical factors for refolding their protein in as little as 1 hour. Unlike many traditional kits, the Quick-FoldTM Kit employs a fractional factorial matrix design that allows the researcher to screen a specific protein in 15 different buffers quickly and easily. Researchers are able to examine a wider range of conditions all in a single experiment, simplifying the process of identifying the best buffer composition and method for the refolding of a given protein. The QuickFoldTM Kit comes with enough buffer for 10 refolding experiments, as well as supplemental Dithiothreitol and a Glutathione Redox System. Each buffer is available for individual purchase and is supplemented with the necessary Glutathione Redox System and/or DTT. Individual Buffers come in 500mL and 1L amounts.

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Kit Components

Buffers 1 - 15	DTT
Glutathione, reduced	Glutathione, oxidized

Order #	Product Name	Type	Unit	Price
0600	QuickFoldTM Protein Refolding Kit	Liquid (ready-to-use)	1 kit	$321.40

Product Specifications

Individual Refolding Buffers	Type	500mL	Price	1L	Price
QuickFoldTM Protein Refolding Buffer 1	Liquid (ready-to-use)	0612-1	$240.40	0613-1	$398.20
QuickFoldTM Protein Refolding Buffer 2	Liquid (ready-to-use)	0612-2	$240.40	0613-2	$398.20
QuickFoldTM Protein Refolding Buffer 3	Liquid (ready-to-use)	0612-3	$240.40	0613-3	$398.20
QuickFoldTM Protein Refolding Buffer 4	Liquid (ready-to-use)	0612-4	$240.40	0613-4	$398.20
QuickFoldTM Protein Refolding Buffer 5	Liquid (ready-to-use)	0612-5	$240.40	0613-5	$398.20
QuickFoldTM Protein Refolding Buffer 6	Liquid (ready-to-use)	0612-6	$240.40	0613-6	$398.20
QuickFoldTM Protein Refolding Buffer 7	Liquid (ready-to-use)	0612-7	$240.40	0613-7	$398.20
QuickFoldTM Protein Refolding Buffer 8	Liquid (ready-to-use)	0612-8	$240.40	0613-8	$398.20
QuickFoldTM Protein Refolding Buffer 9	Liquid (ready-to-use)	0612-9	$240.40	0613-9	$398.20
QuickFoldTM Protein Refolding Buffer 10	Liquid (ready-to-use)	0612-10	$240.40	0613-10	$398.20
QuickFoldTM Protein Refolding Buffer 11	Liquid (ready-to-use)	0612-11	$240.40	0613-11	$398.20
QuickFoldTM Protein Refolding Buffer 12	Liquid (ready-to-use)	0612-12	$240.40	0613-12	$398.20
QuickFoldTM Protein Refolding Buffer 13	Liquid (ready-to-use)	0612-13	$240.40	0613-13	$398.20
QuickFoldTM Protein Refolding Buffer 14	Liquid (ready-to-use)	0612-14	$240.40	0613-14	$398.20
QuickFoldTM Protein Refolding Buffer 15	Liquid (ready-to-use)	0612-15	$240.40	0613-15	$398.20

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Detergent Screening Kit & Individual Detergents

Detergents have been shown to aid in the protein refolding process both of native and recombinant proteins. Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each) for determining the optimum detergent for a given protein refolding scenario. Individual detergents can also be purchased in 100mL quantities.

Kit Components

100 mM Triton-X	100 mM POE(10)L
100 mM Tween 80	100 mM TTAB
100 mM CTAB	100 mM Zwittergent 3-14

Order #	Product Name	Type	Unit	Price
0601	Detergent Screening Kit	Liquid (ready-to-use)	1 kit	$205.40
0603	100 mM Triton-X	Liquid (ready-to-use)	100 mL	$54.50
0604	100 mM Tween 80	Liquid (ready-to-use)	100 mL	$54.50
0605	100 mM CTAB	Liquid (ready-to-use)	100 mL	$54.50
0606	100 mM POE(10)L	100 mL	1 kit	$54.50
0607	100 mM TTAB	Liquid (ready-to-use)	100 mL	$75.20
0608	100 mM Zwittergent 3-14	Liquid (ready-to-use)	100 mL	$349.20

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Cyclodextrin Screening Kit & Individual Cyclodextrins

Cyclodextrins’ unique molecular structure greatly promotes protein refolding. Cyclodextrins can suppress protein aggregation, protect against degradation and loss of protein function. They also bind well to detergents (aggregation suppressors), which allows for easy separation of the detergent from the protein, encouraging proper refolding. Different
cyclodextrins act and respond differently to different protein structures. Because of this, AthenaESTM provides 3 of the most common cyclodextrins in a simple screening kit so that the ideal conditions for refolding a given protein can be found quickly and easily. The Cyclodextrin Screening Kit can be particularly useful when paired with the Detergent Screening Kit. Individual cyclodextrins are also available for purchase.

Kit Components

100 mM β-cyclodextrin	50 mM α-cyclodextrin
100 mM methyl-β-cyclodextrin

Order #	Product Name	Type	Unit	Price
0602	Cyclodextrin Screening Kit	Liquid (ready-to-use)	1 kit	$298.60
0609	100 mM β-cyclodextrin	Liquid (ready-to-use)	100 mL	$175.20
0610	100 mM methyl-β-cyclodextrin	Liquid (ready-to-use)	100 mL	$1,123.10
0611	50 mM α-cyclodextrin	Liquid (ready-to-use)	100 mL	$340.30

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PDQ™ Protease Assay

The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease activity in aqueous samples. The proprietary substrate responds to a wide range of proteases including serine, metallo, aspartate and cysteine proteases such as collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. PDQ™ can be used with just a few simple steps to measure protease activity and requires no centrifugation. The substrate is a cross-linked matrix containing protein substrate and a dye-protein conjugate. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity and can detect nanogram quantities. Each kit is supplied with a trypsin solution for generating standard curves (BAEE equivalent units). PDQ™ is supplied in 48 ready-to-use plasict vials. ​

Product Specifications

Storage	Store at 4°C
Stability at 4 ̊C	3 months from the date of manufacture

Order #	Product Name	Type	Price
0201	PDQ™ Protease Assay	48 Vials	$190.50

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