​ACES™ Promoter Selection Kit

ACES™ (AthenaES™ Complete Expression System): Promoter Selection Kit The ACES™ (AthenaES™ Complete Expression System) Promoter Selection Kit applies the principle that the overall expression rate can determine not only the total accumulation level, but also the amount of soluble protein that is made. By titering the promoter activity, one can fine-tune the expression level to meet the specific needs of target protein production levels.

applicationmanual_aces_promoter.pdf
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Order #	Product Name	Type	Price
0148-1	ACES™ Promoter Selection Kit	1 Kit	$255.20
0149-25	ACES™ pAES25	10µg	$84.70
0150-1	ACES™ Primer A	1 vial	$23.10
0150-2	ACES™ Primer B	1 vial	$23.10
0152-1	ACES™ Inducer Solution A	1mL	$23.10
0152-5	ACES™ Inducer Solution A	5 x 1mL	$93.50
0104	Turbo Broth™	500g	$63.80
0105	Superior Broth™	500g	$67.10
0106	Power Broth™	500g	$55.00
0107	Hyper Broth	500g	$48.40
0108	Glucose M9Y	500g	$38.50
0103	LB (Miller)	500g	$48.40
0109	Glucose Nutrient Mix	150g	$19.80
0123	Augmedium™	100mL	$139.70

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ACES™ Signal Sequence Kit

ACES™ (AthenaES™ Complete Expression System): Signal Sequence Kit The ACES™ (AthenaES™ Complete Expression System) Signal Sequence Kit provides a set of six expression vectors each with a different signal sequence for protein export to the periplasmic space of E. coli using either the SEC, SRP or TAT protein secretion pathway. Since the use of a post translational or co-translational export mechanism is protein-specific and cannot be known a priori, this kit allows for the selection of the translocation pathway best suited for a given recombinant protein. Expression is from the T5 promoter under the control of the lacoperator. Protein production is initiated by the addition of IPTG or lactose (only with Lac+ strains) to the culture medium.

applicationmanual_aces_signal.pdf
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Order #	Product Name	Type	Price
0148-2	ACES™ Signal Sequence Kit	1 Kit	$283.80
0149-30	ACES™ pAES30	10µg	$113.30
0149-31	ACES™ pAES31	10µg	$113.30
0149-32	ACES™ pAES32	10µg	$113.30
0149-33	ACES™ pAES33	10µg	$113.30
0149-34	ACES™ pAES34	10µg	$113.30
0149-35	ACES™ pAES35	10µg	$113.30
0152-1	ACES™ Inducer Solution A	1mL	$23.10
0152-5	ACES™ Inducer Solution A	5 x 1mL	$93.50
0153	ACES™ Inducer Solution B	500 ml	$68.20
0154	ACES™ Secretion Enhancer Solution A	500 ml	$68.20
0155	ACES™ Secretion Enhancer Solution B	500 ml	$68.20
0104	Turbo Broth™	500g	$63.80
0105	Superior Broth™	500g	$67.10
0106	Power Broth™	500g	$55.00
0107	Hyper Broth™	500g	$48.40
0108	Glucose M9Y	500g	$38.50
0103	LB (Miller)	500g	$48.40
0109	Glucose Nutrient Mix	150g	$19.80
0123	Augmendium	100ml	$139.70

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ACES™ YebF Protein Export Kit

ACES™ (AthenaES™ Complete Expression System): YebF Protein Export Kit The ACES™ (AthenaES™ Complete Expression System) YebF Protein Export Kit makes use of the YebF protein to transport other proteins through the inner and outer membranes of E. coli cells. This permits the secretion of target proteins into the culture medium, simplifying downstream purification as well as allowing for the expression of toxic proteins and proteins that do not fold correctly in the E. coli cytoplasm.

applicationmanual_aces_yebf.pdf
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Order #	Product Name	Type	Price
0148-3	ACES™ YebF Protein Export	1 Kit	$283.80
0149-40	ACES™ pAES40	10µg	$141.90
0313	Anti-YebF Antisera	0.5ml	$194.70
0152-1	ACES™ Inducer Solution A	1 ml	$23.10
0152-5	ACES™ Inducer Solution A	5 x 1mL	$93.50
0153	ACES™ Inducer Solution B	500mL	$68.20
0154	ACES™ Secretion Enhancer Solution A	500mL	$68.20
0155	ACES™ Secretion Enhancer Solution B	500mL	$68.20
0104	Turbo Broth™	500g	$63.80
0105	Superior Broth™	500g	$67.10
0106	Power Broth™	500g	$55.00
0107	Hyper Broth™	500g	$48.40
0108	Glucose M9Y	500g	$38.50
0103	LB (Miller)	500g	$48.40
0109	Glucose Nutrient Mix	150g	$19.80
0123	Augmendium	100ml	$139.70

Order

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